In this research line we aim at developing ultrasound (US) image analysis techniques in order to develop tools for image enhancement, lesion detection, biomarkers measurements from 2D, 3D and 4D US images. Currently we address several US images analysis methods in ginecology/obstetrics departments and vascular US image analysis. Example of this is the software package, cIMsT, the Carotid Intima-Media Segmentation and Thickness Measurement System. It was developed at INESC TEC – in cooperation with the Department of Neurology, São João Hospital Center, Portugal. The system accepts as input B-mode longitudinal ultrasound images, automatically segments the far and near media-intima and lumen-intima walls, and outputs several image results and measurements, namely the intima-media thickness and plaque burden. cIMsT provides the possibility of manual adjustments for correcting the automatic segmentation results.